The Hudson River is a 315-mile river that flows from north to south primarily through eastern New York in the United States.
When John Jameson's shuttle landed on the George Washington Bridge, Spider-Man went there to save him and Paul Stevenson. Spider-Man managed to save the two astronauts and the shuttle fell into the Hudson (with Spider-Man still inside). Spider-Man was able to make it to the surface and discovered a black slime on his costume. Spider-Man at first thought that it was polution from the Hudson River. However, he later learned that the black slime was really an alien symbiote.